[Frontiers in Bioscience 6, b17-24, October 1, 2001] 17 DETECTION OF TNF INHIBITHORS (SOLUBLE RECEPTORS) IN THE SERA AND TUMOR CYST FLUID OF PATIENTS WITH MALIGNANT ASTROCYTOMAS OF THE BRAIN
نویسندگان
چکیده
1. Abstract 2. Introduction 3. Materials and methods 3.1. Clinical Characteristics of Patients 3.2. Serum Collection 3.3. Tumor Cyst Fluid Collection 3.4. ELISA for TNF and stnf-r’s 3.5. Cytolytic Bioassay for TNF Activity and TNF Blocking Factor Activity 3.6. Immunohistochemical Staining of Astrocytoma Tissue Sample 3.7. Statistical Analysis 4. Results 4.1. Serum Levels of TNF and the 55 kda and 75 kda stnf-R’s in Malignant Astrocytoma Patients and Normal Individuals 4.2. TNF Levels in Brain Tumor Cyst Fluid Samples 4.3. Levels of the 55 and 75 kda stnf-r’s, and TNF Blocking Factor Activity in the Tumor Cyst Fluid From Patients With Malignant Astrocytomas 4.4. Immunohistochemical Staining of Astrocytoma Tissue 5. Discussion 6. Acknowledgements 7. References
منابع مشابه
Evaluation of Soluble Toll-Like Receptors 2, 4, 9 and Their Damps Signaling Molecules (HMGB1 & HSP70) in Breast Cancer Patients of Basrah Province
Introduction: Toll-Like Receptors (TLRs) are members of pattern recognition receptors that recognize various molecules, including pathogen-associated molecular patterns and dead-associated molecular patterns. These receptors are expressed by immune, non-immune, and tumor cells. Some TLRs are implicated in tumor progression, while others are involved in tumor suppression. Our study aimed to eval...
متن کاملHigher serum levels of tumour necrosis factor and its soluble receptors are associated with ovarian tumours
INTRODUCTION Tumour necrosis factor (TNF) and its soluble receptors type 1 (sTNF-R1) and type 2 (sTNF-R2) have been suggested as key mediators between apoptosis and cancer cell progression. The aim was to examine concentrations of the parameters in the serum of women with ovarian tumour and in the fluid from ovarian cysts of women with serous cystadenoma. MATERIAL AND METHODS The study includ...
متن کاملAntioxidants and Proinflamatory Cytokines in the Sera of Patients with Cutaneous Leishmaniasis
Background: Leishmania is a significant health problem in many parts of the world. Tumor necrosis factor (TNF) plays an essential role in Leishmania major infections. Objective: To study the pro-inflammatory cytokines and antioxidants in four groups of cutaneous leishmaniasis patients. Methods: 39 patients were divided into four groups of: 1) active (acute phase of treatment); 2) non-healing (r...
متن کاملTNF-alpha, IL-6 and their soluble receptor serum levels and secretion by neutrophils in cancer patients.
Simultaneous evaluation of cytokines and their soluble receptor production and the serum levels can be helpful in understanding the local and systemic immune response of a tumor-bearing host. In the present study we examined serum levels of TNF-alpha, IL-6 and their soluble receptors: sTNFRp55, sTNFRp75 and sIL-6R confronted with their production by the polymorphonuclear neutrophils (PMN) from ...
متن کاملSerum Level of HER-2 Extracellular Domain in Iranian Patients with Breast Cancer: A Follow-up Study
Background: A soluble form of HER-2/neu extracellular domain (sHER-2) is reported to be released in the sera of metastatic breast cancer patients. Objective: To measure the level of sHER-2 in sera of 115 breast cancer patients. Methods: Serial samples of 27 patients with metastasis, 18 non-metastatic patients, 15 patients in stage 0/I and 14 patients with accompanying benign breast disease were...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2002